I know this is probably common sense for most of you, but neglecting to check my tire sealant bit me in the ass today. The weather was perfect and I escaped from work early to hit the trails. Everything was going great until my tire went flat 5 miles into the trail. I tired to air it back up and in the process found a hole right in the center of the tire tread. I pointed the hole down, bit nothing happened. Air leaked from the hole as fast as ever. I spun the wheel around a bit, but nothing. No sealant.
I ended up throwing a tube in and in the process confirmed that there was no more sealant in the tire. It must have all been used up on previous punctures (I find sealant on the frame and tire pretty often). And to add insult to injury, a mile down the trail the tube got a hole from a goat head (there is a lot of thorns and the like out here in AZ). I ended up using my last cartridge and a borrowed one from a coworker to limp back. Now I'm at there car waiting while they're out there enjoying the heck out of this great weather.
Check your sealant early and often! I'm just glad this didn't happen on a race!